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FiberPrep® Cooler Box (DNA Extraction Kit) - 100 Extractions
EXT-002
The FiberPrep® Cooler Box kit delivers high quality DNA solutions for the combing procedure from fresh blood samples or cultured cells.
More info
The protocol is used to purify and store genomic DNA molecules protected from mechanical stress. After embedding cells in an agarose plug, proteins are digested by a proteinase and cell membranes solubilized by a surfactant, under inhibition of DNase activity. The treated DNA plugs are then available for preparation of a high molecular weight DNA solution.
The kit do not includes the agarase enzyme and PBS trypsine.
- Results in solutions of very high molecular weight DNA (100 kbp to 2-3 Mbp)
- Designed for pelleted cells from cell cultures or WBC primary samples
- The extraction protocol can be adapted for various organisms (yeast, plants…)
