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FiberPrep® (DNA Extraction Kit) - 10 Extractions
EXTR-001-10
The FiberPrep® kit delivers high quality DNA solutions for the combing procedure from fresh blood samples or cultured cells.
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The protocol is used to purify and store genomic DNA molecules protected from mechanical stress. After embedding cells in an agarose plug, proteins are digested by a proteinase and cell membranes solubilized by a surfactant, under inhibition of DNase activity. The treated DNA plugs are then available for preparation of a high molecular weight DNA solution by agarase digestion.
- Results in solutions of very high molecular weight DNA (100 kbp to 2-3 Mbp)
- Designed for pelleted cells from cell cultures or WBC primary samples
- The extraction protocol can be adapted for various organisms (yeast, plants…)
